CBF | Lecture 52
CBF | Lecture 52
AI Mind Map
Interactive visualization of lecture concepts
Generated 1 month ago
Files & Documents
1 file available for download
Focus
Quiz Questions (5 questions)
1. Synthetic analogs of nucleotides are used in the treatment of cancer.
2. Mutations in mitochondrial DNA result in a maternal pattern of diseases.
3. Mutations in mitochondrial DNA result in defects in enzymes of Respiratory chain I and IV.
4. Mutations in mitochondrial DNA result in Non-Mendelian inheritance for diseases.
5. In a DNA molecule, if the thymine concentration is 30%, the guanosine concentration will be 20%.
Previous Exam Questions (46 questions)
1. For DNA sequencing, DNA must be in its primary structure.
2. Mutations in mitochondrial DNA result in a maternal pattern of diseases and non-Mendelian inheritance, and can cause defects in enzymes of Respiratory chain I and IV, but do not cause sickle cell anemia.
3. The predominant form of DNA under physiological conditions is the B-form.
4. Mitochondrial DNA (mtDNA) is a double-stranded circular DNA and is maternally inherited.
5. The width of the DNA double helix in the B-form is 1.9 nm.
6. Histone H1 is not part of the histone octamer, which consists of H2A, H2B, H3, and H4.
7. The S phase of the cell cycle involves DNA replication.
8. Methotrexate targets the S phase of the cell cycle.
9. Hydrogen bonds hold the two strands of DNA together.
10. In Chargaff’s rule, if a DNA sample has 15% adenine, the percentage of cytosine will be 35%.
11. The backbone of a DNA strand is composed of phosphates and deoxyribonucleosides.
12. Euchromatin is loosely packed and transcriptionally active.
13. The primary function of the nucleosome in chromatin is to condense and stabilize DNA.
14. DNA denaturation is the process where DNA strands separate without breaking covalent bonds when heated.
15. Errors in DNA replication due to a failure in correcting mismatched base pairs likely occur in the G2 phase of the cell cycle.
16. During a Southern blot, complementary DNA sequences are bound together through a process called hybridization.
17. If a sample of DNA was analyzed, and 20% of its bases were found to be cytosine, then according to Chargaff's rule, the percentage of adenine in this sample is 30%.
18. Vincristine, a drug used in chemotherapy, targets the M phase of the cell cycle.
19. The types of DNA forms are B-form (right-handed, 1.9 nm width, 10 bp/turn, physiological conditions), A-form (right-handed, wider (2.3 nm), condensed, high salt medium, 11 bp/turn), and Z-form (left-handed, elongated (1.8 nm width), zig-zag phosphodiester backbone).
20. The components of a nucleosome are DNA wrapped around an octamer of histones (H2A, H2B, H3, H4)₂ and linker DNA bound by H1 histone.
21. The levels of chromatin condensation are nucleosome (10 nm fiber), chromatin fiber (30 nm), chromatids, and chromosomes.
22. Agents targeting the S phase of the cell cycle include Methotrexate, Hydroxyurea, and 5-Fluorouracil.
23. The components of chromatin are double-stranded DNA, RNA (small amount), and histone proteins.
24. For DNA sequencing, DNA must be in its primary structure.
25. Mutations in mitochondrial DNA result in a maternal pattern of diseases, non-Mendelian inheritance for diseases, and defects in enzymes of Respiratory chain I and IV, but not sickle cell anemia.
26. The predominant form of DNA under physiological conditions is the B-form.
27. A child presenting with seizures and lactic acidosis may have a mitochondrial DNA (mtDNA) disorder, which is characterized by double-stranded circular DNA inherited maternally.
28. The width of the DNA double helix in the B-form is 1.9 nm.
29. Histone H1 is not part of the histone octamer; the histone octamer consists of H2A, H2B, H3, and H4.
30. DNA replication occurs during the S phase of the cell cycle.
31. Methotrexate targets the S phase of the cell cycle.
32. Hydrogen bonds hold the two strands of DNA together.
33. In Chargaff’s rule, if a DNA sample has 15% adenine, the percentage of cytosine will be 35%.
34. The backbone of a DNA strand is composed of phosphates and deoxyribonucleosides.
35. Euchromatin is loosely packed and transcriptionally active.
36. The primary function of the nucleosome in chromatin is to condense and stabilize DNA.
37. When a researcher heats a DNA sample and observes that the strands separate without breaking covalent bonds, this process is referred to as denaturation.
38. A patient with a genetic disorder who has errors in DNA replication due to a failure in correcting mismatched base pairs likely has an error in the G2 phase of the cell cycle.
39. During a Southern blot, complementary DNA sequences are bound together in a process called hybridization.
40. If a sample of DNA was analyzed, and 20% of its bases were found to be cytosine, then using Chargaff's rule, the percentage of adenine in this sample is 30%.
41. A chemotherapy patient treated with vincristine is receiving a drug targeting the M phase of the cell cycle.
42. The types of DNA forms are B-form, which is right-handed, 1.9 nm wide, and has 10 base pairs per turn under physiological conditions; A-form, which is right-handed, wider (2.3 nm), condensed, and has 11 base pairs per turn in a high salt medium; and Z-form, which is left-handed, elongated (1.8 nm width), and has a zig-zag phosphodiester backbone.
43. The components of a nucleosome are DNA wrapped around an octamer of histones (H2A, H2B, H3, H4)₂ and linker DNA bound by H1 histone.
44. The levels of chromatin condensation are the nucleosome (10 nm fiber), the chromatin fiber (30 nm), chromatids, and chromosomes.
45. Agents targeting the S phase of the cell cycle include methotrexate, hydroxyurea, and 5-Fluorouracil.
46. The components of chromatin are double-stranded DNA, RNA (in small amounts), and histone proteins.
Content processed on Dec 15, 2025 8:14 PM
Slide Explanations
CBFLecture52
Lecture Summary
📚 AI-Generated Summary
Comprehensive analysis of lecture content
Okay, look man, let me break down DNA structure, chromatin organization, and the cell cycle for you. This is really important stuff, and I’ll try to keep it simple. **DNA Structure and Organization** DNA, deoxyribonucleic acid, is the molecule that carries genetic information in all living organis...
Flashcards
Total Cards
9
Due for Review
-
Mastered
-
Quick Preview
Define the terms nucleoside and nucleotide.
A nucleoside is a nitrogenous base (Adenine, Guanine, Cytosine, Thymine, or Urac...
What are some chemotherapeutic agents and the specific phase...
Chemotherapeutic agents target specific phases of the cell cycle: * **S-phase:...
Describe the composition of a nucleotide, including the spec...
A nucleotide consists of: * A nitrogenous base (either a purine or a pyrimidi...
Available Quizzes
Quiz for CBF | Lecture 52
12 QuestionsAI-generated quiz for CBF | Lecture 52
This may take a few minutes